![]() ![]() Western blot specificity was 100% when antibody reactivity with different antigenic bands was analyzed and associated. ELISA sensitivity improved from 85% to 92% when the Western blot results were used. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85% sensitivity and 91% specificity. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. Phone: (55) 220 recombinant antigens and a crude bacterial antigen of a wild M. IVBiolab-Merieux S/A and Tropical Medicine Institute, São Paulo, SP, Brazilĭepartamento de Análises Clínicas e Toxicológicas IIClinical Hospital Faculty of Medicine of São Paulo University, São Paulo IFederal University of Santa Maria, Santa Maria, RS tuberculosis recombinant antigens diagnosisĬombined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis The association of TbF6/DPEP antigens used in ELISA with specific patterns of reactivity determined by Western blot can help make an identification when classic methods for the diagnosis of pulmonary tuberculosis are not sufficient. ![]() ![]() Two recombinant antigens and a crude bacterial antigen of a wild M. ![]()
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